primary antibodies against klotho  (Thermo Fisher)

Journal: Immunity & Ageing : I & A

Article Title: Impact of Klotho genotype on lactylation in Alzheimer’s disease and mechanistic insights

doi: 10.1186/s12979-026-00563-x

Figure Lengend Snippet: Klotho genotypes regulate lactylation and inflammation in Aβ/IBO−induced primary microglia. A Relative mRNA expression of Klotho measured by qRT − PCR. B Representative Western blot images and quantification of L−Lactyl, H4K12la, Klotho, and Aβ protein levels (Histone H4 and GAPDH were used as loading controls, as indicated). C Concentrations of TNF − α, IL−1β, iNOS, and IL − 10 assessed by ELISA. Data are presented as mean ± SD ( n = 3). Statistical significance: * p < 0.05; *** p < 0.001; # p < 0.05; ## p < 0.01; ### p < 0.001; & p < 0.05; && p < 0.01; &&& p < 0.01; ^^ p < 0.01; ^^^ p < 0.001

Article Snippet: Thereafter, we incubated the sections with cover slips overnight at 4°C in a solution containing 5% goat serum and primary antibodies against Klotho (Bioss, bs-2925R), Aβ (ABclonal, A24949 ), H4K12la (Immunoway, YK0013, Plano, USA), and L-lactyl (PTM, PTM-1401RM).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Immunity & Ageing : I & A

Article Title: Impact of Klotho genotype on lactylation in Alzheimer’s disease and mechanistic insights

doi: 10.1186/s12979-026-00563-x

Figure Lengend Snippet: Klotho genotypes regulate lactylation and inflammation in Aβ/IBO−induced BV − 2 microglial cells. A Relative mRNA expression of Klotho measured by qRT − PCR. B Representative Western blot images and quantification of L−Lactyl, H4K12la, Klotho, and Aβ protein levels (Histone H4 and GAPDH were used as loading controls, as indicated). ,. C Concentrations of TNF − α, IL−1β, iNOS, and IL − 10 assessed by ELISA. Data are presented as mean ± SD ( n = 3). Statistical significance: * p < 0.05; *** p < 0.001; # p < 0.05; ## p < 0.01; ### p < 0.001; & p < 0.05; && p < 0.01; &&& p < 0.01; ^^ p < 0.01; ^^^ p < 0.001

Article Snippet: Thereafter, we incubated the sections with cover slips overnight at 4°C in a solution containing 5% goat serum and primary antibodies against Klotho (Bioss, bs-2925R), Aβ (ABclonal, A24949 ), H4K12la (Immunoway, YK0013, Plano, USA), and L-lactyl (PTM, PTM-1401RM).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Immunity & Ageing : I & A

Article Title: Impact of Klotho genotype on lactylation in Alzheimer’s disease and mechanistic insights

doi: 10.1186/s12979-026-00563-x

Figure Lengend Snippet: Transcriptomic profiling reveals genotype−specific responses to Klotho in Aβ/IBO−treated BV − 2 cells. A Volcano plot of differentially expressed genes (DEGs) between Klotho WT and F352V groups, up−regulated genes are represented by red dots, down−regulated genes by blue dots, and indifferent genes by gray dots. B Heatmap showing hierarchical clustering of DEGs. C GO enrichment analysis of DEGs by biological process, cellular component, and molecular function. D KEGG pathway enrichment analysis. E Reactome pathway enrichment analysis. F Disease Ontology (DO) association analysis. G Protein–protein interaction (PPI) network of DEGs. DEGs were defined by |log₂FoldChange| > 1 and p < 0.05

Article Snippet: Thereafter, we incubated the sections with cover slips overnight at 4°C in a solution containing 5% goat serum and primary antibodies against Klotho (Bioss, bs-2925R), Aβ (ABclonal, A24949 ), H4K12la (Immunoway, YK0013, Plano, USA), and L-lactyl (PTM, PTM-1401RM).

Techniques:

Journal: Immunity & Ageing : I & A

Article Title: Impact of Klotho genotype on lactylation in Alzheimer’s disease and mechanistic insights

doi: 10.1186/s12979-026-00563-x

Figure Lengend Snippet: Klotho genotypes modulate OSM/JAK1/STAT3 signaling and lactylation in primary microglia. A mRNA expression levels of OSM, JAK1, STAT3 and Klotho detected by qRT − PCR. B Protein levels of OSM, JAK1, STAT3, p−STAT3, L−Lactyl and H4k12la detected by Western blot. Data are presented as mean ± SD ( n = 3). Statistical significance: * p < 0.05; *** p < 0.001; # p < 0.05; ## p < 0.01; ### p < 0.001; & p < 0.05; && p < 0.01; &&& p < 0.01; ^^ p < 0.01; ^^^ p < 0.001

Article Snippet: Thereafter, we incubated the sections with cover slips overnight at 4°C in a solution containing 5% goat serum and primary antibodies against Klotho (Bioss, bs-2925R), Aβ (ABclonal, A24949 ), H4K12la (Immunoway, YK0013, Plano, USA), and L-lactyl (PTM, PTM-1401RM).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Immunity & Ageing : I & A

Article Title: Impact of Klotho genotype on lactylation in Alzheimer’s disease and mechanistic insights

doi: 10.1186/s12979-026-00563-x

Figure Lengend Snippet: Klotho genotypes affect Aβ accumulation and cytokine expression in primary microglia. A Immunofluorescence detection of Klotho and Aβ in primary microglia. B Concentrations of TNF − α, IL−1β, iNOS, and IL − 10 assessed by ELISA. Data are presented as mean ± SD ( n = 3). Statistical significance: ** p < 0.01; *** p < 0.001; ### p < 0.001; &&& p < 0.001; ^^^ p < 0.001

Article Snippet: Thereafter, we incubated the sections with cover slips overnight at 4°C in a solution containing 5% goat serum and primary antibodies against Klotho (Bioss, bs-2925R), Aβ (ABclonal, A24949 ), H4K12la (Immunoway, YK0013, Plano, USA), and L-lactyl (PTM, PTM-1401RM).

Techniques: Expressing, Immunofluorescence, Enzyme-linked Immunosorbent Assay

Journal: Immunity & Ageing : I & A

Article Title: Impact of Klotho genotype on lactylation in Alzheimer’s disease and mechanistic insights

doi: 10.1186/s12979-026-00563-x

Figure Lengend Snippet: Klotho genotypes influence OSM/JAK1/STAT3 signaling and lactylation in BV − 2 cells. A mRNA expression levels of OSM, JAK1, STAT3 and Klotho detected by qRT − PCR. B Protein levels of OSM, JAK1, STAT3, p−STAT3, L−Lactyl and H4k12la detected by Western blot. Data are presented as mean ± SD ( n = 3). Statistical significance: * p < 0.05; *** p < 0.001; ### p < 0.001; & p < 0.05; && p < 0.01; &&& p < 0.01; ^ p < 0.05; ^^ p < 0.01; ^^^ p < 0.001

Article Snippet: Thereafter, we incubated the sections with cover slips overnight at 4°C in a solution containing 5% goat serum and primary antibodies against Klotho (Bioss, bs-2925R), Aβ (ABclonal, A24949 ), H4K12la (Immunoway, YK0013, Plano, USA), and L-lactyl (PTM, PTM-1401RM).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Immunity & Ageing : I & A

Article Title: Impact of Klotho genotype on lactylation in Alzheimer’s disease and mechanistic insights

doi: 10.1186/s12979-026-00563-x

Figure Lengend Snippet: Klotho genotypes affect Aβ accumulation and cytokine release in BV − 2 microglial cells. A Immunofluorescence detection of Klotho and Aβ expression in BV − 2 cells. B , Concentrations of TNF − α, IL−1β, iNOS, and IL − 10 assessed by ELISA. Data are presented as mean ± SD ( n = 3). Statistical significance: * p < 0.05; *** p < 0.001; ### p < 0.001; &&& p < 0.001; ^ p < 0.05; ^^ p < 0.01; ^^^ p < 0.001

Article Snippet: Thereafter, we incubated the sections with cover slips overnight at 4°C in a solution containing 5% goat serum and primary antibodies against Klotho (Bioss, bs-2925R), Aβ (ABclonal, A24949 ), H4K12la (Immunoway, YK0013, Plano, USA), and L-lactyl (PTM, PTM-1401RM).

Techniques: Immunofluorescence, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Immunity & Ageing : I & A

Article Title: Impact of Klotho genotype on lactylation in Alzheimer’s disease and mechanistic insights

doi: 10.1186/s12979-026-00563-x

Figure Lengend Snippet: OSM knockdown improves cognitive performance and pathology in APP/PS1 mice. A Spatial learning and memory assessed by Morris water maze before lentiviral injection. B Spatial learning and memory evaluated after injection of Klotho and/or sh − OSM lentivirus. C , Hematoxylin and eosin (H&E) staining of hippocampal sections. D Immunohistochemical detection of Klotho and Aβ expression in mouse brain. E Enlarged views of Aβ immunohistochemical staining from the frams. Data are presented as mean ± SD ( n = 3). Statistical significance: * p < 0.05; *** p < 0.001; ### p < 0.001; &&& p < 0.001; ^ p < 0.05; ^^^ p < 0.001

Article Snippet: Thereafter, we incubated the sections with cover slips overnight at 4°C in a solution containing 5% goat serum and primary antibodies against Klotho (Bioss, bs-2925R), Aβ (ABclonal, A24949 ), H4K12la (Immunoway, YK0013, Plano, USA), and L-lactyl (PTM, PTM-1401RM).

Techniques: Knockdown, Injection, Staining, Immunohistochemical staining, Expressing

Journal: Immunity & Ageing : I & A

Article Title: Impact of Klotho genotype on lactylation in Alzheimer’s disease and mechanistic insights

doi: 10.1186/s12979-026-00563-x

Figure Lengend Snippet: Molecular effects of OSM knockdown in APP/PS1 mouse brain. A mRNA expression levels of Klotho, OSM, JAK1, and STAT3 measured by qRT − PCR. B Protein levels of OSM, AK1, STAT3, p−STAT3, L−Lactyl and H4k12la detected by Western blot. C Concentrations of TNF − α, IL−1β, iNOS, and IL − 10 assessed by ELISA. C Expression levels of were detected by ELISA. Data are presented as mean ± SD ( n = 3). Statistical significance: * p < 0.05; *** p < 0.001; ### p < 0.001; && p < 0.01; &&& p < 0.001; ^ p < 0.05; ^^ p < 0.01; ^^^ p < 0.001

Article Snippet: Thereafter, we incubated the sections with cover slips overnight at 4°C in a solution containing 5% goat serum and primary antibodies against Klotho (Bioss, bs-2925R), Aβ (ABclonal, A24949 ), H4K12la (Immunoway, YK0013, Plano, USA), and L-lactyl (PTM, PTM-1401RM).

Techniques: Knockdown, Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Animals : an Open Access Journal from MDPI

Article Title: Characterization of Urine-Derived Stromal/Stem Cells from Healthy Dogs and Dogs Affected by Chronic Kidney Disease (CKD)

doi: 10.3390/ani15020242

Figure Lengend Snippet: Klotho protein expression was confirmed by Western blotting. Results are shown as the mean ± SEM. * p < 0.05. Legend: hUSCs = USCs from healthy dogs; sUSCs: = USCs from sick dogs.

Article Snippet: The membrane was cut into two pieces: one was hybridized with 1 μg of mouse monoclonal primary antibody against Klotho protein (sc-515942 Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and the other with 1 μg of anti GAPDH (sc-47724 Santa Cruz Biotechnology, Inc., Dallas, TX, USA) by the SNAP i.d.

Techniques: Expressing, Western Blot

Journal: Physiological Reports

Article Title: Fibroblast growth factor‐23 and parathyroid hormone suppress small intestinal magnesium absorption

doi: 10.14814/phy2.15247

Figure Lengend Snippet: The FGFR1 and PTHR1 protein expression in duodenum, jejunum, and ileum of Sprague‐Dawley rats (a). The Klotho protein expression (b) in duodenum, jejunum, ileum, and kidney of Sprague‐Dawley rats

Article Snippet: The membrane was probed with 1:1000 primary antibodies raised against CNNM4 (catalog no. SC‐68437; Santa Cruz Biotechnology, Santa Cruz, CA, USA), TRPM6 (catalog no. PA5‐77326; Thermo Fisher Scientific Inc.), PTHR1 (catalog no. MBS9706527; MyBioSource), FGFR1 (catalog no. ab10646; Abcam, Cambridge, UK), Klotho (catalog no. PA5‐21078; Thermo Fisher Scientific Inc), or β‐actin (catalog no. ab8226; Abcam).

Techniques: Expressing

Journal: Bioengineered

Article Title: MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4

doi: 10.1080/21655979.2021.1871817

Figure Lengend Snippet: RIPK4 was a direct target of miR-330-3p in ES-2 cells. (a) The binding sites between RIPK4 and miR-330-3p predicted by TargetScan. (b)The binding relationship of RIPK4 to miR-330-3p validated by dual-luciferase reporter gene assay, * P < 0.05. The expression level of RIPK4 was detected using RT-qPCR (c) and western blot (d-e), * P < 0.05

Article Snippet: Primary antibodies against RIPK4 (ab203541, Abcam, Cambridge, UK) and GAPDH (ab8245, Abcam, Cambridge, UK) were utilized in this study.

Techniques: Binding Assay, Luciferase, Reporter Gene Assay, Expressing, Quantitative RT-PCR, Western Blot

Journal: Bioengineered

Article Title: MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4

doi: 10.1080/21655979.2021.1871817

Figure Lengend Snippet: Overexpression of RIPK4 antagonized the effects of miR-330-3p on ES-2 cells. The inhibition effect of miR-330-3p on RIPK4 expression was rescued by RIPK4 overexpression detected using RT-qPCR (a) and western blot (b), * P < 0.05. The inhibition effect of miR-330-3p on cell proliferation, migration and invasion was partly reversed by RIPK4 overexpression detected using MTT (c) and transwell assay(d), * P < 0.05

Article Snippet: Primary antibodies against RIPK4 (ab203541, Abcam, Cambridge, UK) and GAPDH (ab8245, Abcam, Cambridge, UK) were utilized in this study.

Techniques: Over Expression, Inhibition, Expressing, Quantitative RT-PCR, Western Blot, Migration, Transwell Assay

Journal: Bioengineered

Article Title: MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4

doi: 10.1080/21655979.2021.1871817

Figure Lengend Snippet: The expression of RIPK4 was inversely correlated with miR-330-3p expression in OV tissues

Article Snippet: Primary antibodies against RIPK4 (ab203541, Abcam, Cambridge, UK) and GAPDH (ab8245, Abcam, Cambridge, UK) were utilized in this study.

Techniques: Expressing